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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and therefore may perhaps add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial role in endorsing mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants have been reported but specific nucleotide sequence details is not nevertheless offered.
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Blocking peptides are peptides that bind exclusively to the goal antibody and block antibody binding. These peptide normally incorporates the epitope identified through the antibody.
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This guideline outlines even more achievable optimization of cellular and immunoassay parameters to be certain the absolute best final results are...
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This guide outlines even further achievable optimization of mobile and immunoassay parameters to make certain the very best results are...
Antibodies bound to the blocking peptide now not bind towards the epitope on the target protein. By evaluating the staining with the blocked antibody vs . the antibody by yourself, you can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins functionality as regulators of CDK kinases. Different cyclins show unique expression and degradation designs which lead for the here temporal coordination of each mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is also degraded as cells progress as a result of S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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